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> micro-Flask(細菌/細胞株篩選/懸浮培養條件測試用)

micro-Flask(細菌/細胞株篩選/懸浮培養條件測試用)

產品介紹：

An integrated, easy-to-use, and cost-effective technology platform for the rapid handling and growth of large numbers of microbial strains, clonal libraries, mutant banks, and cell lines in 6, 24 or 96-well microtiter plates.The system enables a single person to grow and test thousands of strains simultaneously with a minimum of repetitive handlings: all handling is performed in parallel for sets of 6, 24 or 96 strains.More efficient use of your current orbital shaker: time savings up to a factor 10 possible.The Micro-Flask system consists of a specialized microtiter plate closures (“sandwich covers”) and clamps to hold the closures in place. Micro-Flasks are designed to provide a headspace refreshment rate of 1-2 working volumes per minute in orbial shakers, permitting oxygen concentrations of at least 18% (v/v), even when oxygen uptake rates are as high as 40 mmol O2 L-1 h-1. Evaporation at these conditions is kept at a minimum (as low as 2% per day), which permits culture times that are weeks long.The sandwich covers consist of sterilizable, re-usable layers that provide a positive seal on each well of the plate (thus preventing cross-contamination and eliminating well-to-well variability), promote gas transfer, slow evaporation, and prevent contamination.

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規格訊息：

micro-Flask

A mature, easy-to-use, and cost-effective technology platform for the high-quality cultivation in microplates of large numbers of microbial strains, clonal libraries, mutant banks, CHO-cells, etc.

In comparison to Erlenmeyer flasks, the same quality in terms of mixing, oxygen-transfer rates and reproducibility is readily achieved, provided the right orbital shaking conditions are applied.

- High-throughput screening for new enzyme activities or formation of secondary metabolites (heterogeneous culture collections)
-  Cultivation of CHO cells and other animal cell lines
-  Screening and distribution of clone libraries in E. coli or yeasts
-  High-throughput screening for high-activity or high-productivity mutants
-  Comparative studies e.g. clinical isolates
-  Growth medium optimization studies

6 & 24 & 96 deepwell

品牌: by Enzyscreen

	Product No.		square / deep wells	Volume per well	Recommended culture volume per well	typical shaking conditions	packed
square 6-deepwell	CR1406	Polystyrene (PS) single use (non-autoclavable)	34x34 mm/ 80 mm	82 ml	3-35 ml	180 rpm/50 mm 225 rpm/25 mm	4/box (gamma irradiated)
square 24-deepwell	CR1424	Polypropylene	17x17 mm/ 40 mm	11 ml	2.5-8 ml	225 rpm/50 mm 300 rpm/25 mm	1/pkg or 8/box autoclavable (with sandwich cover on top, if desired)
square 24-deepwell	CR1424cl	Polystyrene (PS) single use (non-autoclavable)	17x17 mm/ 40 mm	11 ml	2.5-8 ml	225 rpm/50 mm 300 rpm/25 mm	8/box(gamma irradiated)
square 96-deepwell	CR1496b	Polypropylene	8x8 mm/ 40 mm	2.2 ml	0.5-0.75 ml	300 rpm/50 mm	1/pkg or 8/box autoclavable (with sandwich cover on top, if desired)

sandwich covers

have 4 or 5 layers(all re-usable and autoclavable):

A: a stainless steel lid for rigidity

B: a microfiber filter (blue)

C: an ePTFE filter (0.3 micron) laminated between two polyester/polyamide fabrics (green)

D: a stainless steel foil with pinholes to control the headspace refreshment rate and evaporation(only in covers CR1596 and CR1524a)

E: a silicone layer with 96 holes (to hermetically seal the "mini-reactors")

The most critical aspect of microbial and cell growth in microtiter plates is the closure system of the individual wells.

The closure system must:

•prevent (cross)-contamination even during vigorous shaking

•permit a reproducible and defined exchange rate of headspace air

•limit evaporation.

•Equal physical conditions in all wells is a further requirement: the wells in the corners should have exactly the same characteristics as the wells in the centre of the microtiter plate.

Inspired by the general principle used for multi-litre bioreactors (an average aerobic microbial culture needs an air supply of one working volume per minute), we designed the standard sandwich covers (for fast growing bacteria) to have a headspace refreshment rate of 1-2 culture volumes per minute.

Such a headspace refreshment rates secures oxygen concentrations in the headspace of at least 18% (v/v), even at an oxygen consumption rate by the culture of 40 mmol O2/L/ minute. Such a headspace refreshment rate assures a sufficient oxygen supply, but limits the evaporation to about 2% of the culture volume per day (at an ambient humidity of 50% and 30ºC, see hydrodynamics, oxygen transfer rates) this degree of water loss is acceptable for most projects, and so avoids the need for air humidification in the incubator for most applications. The latter additionally prevents fungal growth in the incubator and so contributes to a safe working environment. To increase the gas-liquid transfer, orbital shaking is our method of choice since it is proven to be effective, and gives rise to exactly the same hydrodynamic conditions in each well. For slow growing strains and (animal) cell cultures, we supply sandwich covers with slower headspace exchange rates (in the order of 0.2 culture volumes per minute). This allows cultivation times of 5-14 days without excessive evaporation.

	Sandwich cover	Product No	size holes in solid silicone layer	exchange of headspace air	evaporation rate (30ºC, 50% humidity)	purpose	packed
videos 6 deepwell MTPs	6 deepwell MTPs	CR1206	7 x 5 mm	7.5 ml / minute	150 µl/well/day	1-5 days cultivations	1/pkg
videos 6 deepwell MTPs	6 deepwell MTPs	CR1206a (low-evaporation version of CR1206)	4 x 5 mm	7.5 ml / minute	150 µl/well/day	3-15 days cultivations	1/pkg
videos 24 deepwell MTPs	24 deepwell MTPs	CR1224	2.5 x 5 mm	2.5 ml / minute	50 µl/well/day	1-5 days cultivations	1/pkg
videos 24 deepwell MTPs	24-square deepwell MTPs	CR1224e (Low-evaporation version of CR1224d)	1.2 x 5 mm	0.7 ml / minute	16 µl/well/day	3-14 days cultivations	1/pkg
videos 96 deepwell MTPs	96 deepwell MTPs	CR1296	1.5 x 5 mm	1 ml / minute	22 µl/well/day	1-5 days cultivations	1/pkg
videos 96 deepwell MTPs	96 deepwell MTPs	CR1296a: (Low-evaporation version of CR1296)	0.8 x 5 mm	0.3 ml / minute	8 µl/well/day	3-14 days	1/pkg

Clamps

	Product No.	規格	packed
4 position clamp	CR1700	only for deepwell plates +covers fits up to 4 deepwell MTPs	1/Set
2 position clamp	CR1702	only for deepwell plates +covers fits up to 2 deepwell MTP	1/Set
4 position Universal clamp	CR1800	for all types of plates fits up to 16 shallow well MTPs, 12 half deepwell MTPs or 4 deepwell MTPs	1/Set
2 position Universal clamp	CR1600	for all types of plates fits up to 8 shallow well MTPs, 6 half deepwell MTPs or 2 deepwell MTPs	1/Set