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Parameters to Increase Rate of Dialysis

Sample & Buffer Concentrations

21. equal except for retained and transferred species

Sample to Dialysate Ratio

21. 1 : 1000+ by volume

Membrane MWCO

21. 90-120% of the retained species molecular size

Tubing Length (Surface Area : Volume Ratio)

21. 30 cm ( 0.1 – 1ml / cm)

Temperature

21. Elevated (36-60 Celsius)

Dynamic Dialysis

Buffer Changing

21. 4-5 changes at 2, 4, 6-8, 8-10, 14-16 & 20 hours

Duration (Resulting)

21. 0.5 – 1.5 days