請問 - 該如何縮短Dialysis時間?提升效率!

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Parameters to Increase Rate of Dialysis

Sample & Buffer Concentrations

u  equal except for retained and transferred species

Sample to Dialysate Ratio

u  1 : 1000+ by volume

Membrane MWCO

u  90-120% of the retained species molecular size

Tubing Length (Surface Area : Volume Ratio)

u  30 cm ( 0.1 – 1ml / cm)

Temperature

u  Elevated (36-60 Celsius)

Dynamic Dialysis

Buffer Changing

u  4-5 changes at 2, 4, 6-8, 8-10, 14-16 & 20 hours

Duration (Resulting)

u  0.5 – 1.5 days


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